Similarly, the erythroleukemia line K demonstrated no significant 51 Cr release or alteration in subsequent proliferative rate date not shown upon exposure to micromolar or lower concentrations of Leu-Leu-OMe.
NK activity has been shown to be augmented after exposure to interferons Gidlund et al. Graft versus host disease GVHD is a major problem in allogeneic bone marrow transplantation. In addition, the analysis of the pharmacological activity data revealed that cycloheptapeptide 8 displayed more bioactivity against the pathogenic microbes and cell lines when compared to its linear form 7.
At room temperature, it is most stable at pH 4. Additional studies are required to evaluate whether the use of aspartame as a sugar substitute might not in turn result in an increased human consumption of D-aspartic acid and D-phenylalanine.
V 72, p ; Podack et al.
The current studies were undertaken to determine whether treatment of the donor inoculum with the anticytotoxic cell compound L-leucyl-L-leucine methyl ester Leu-Leu-OMe would alter the development of GVHD in a murine model.
V 49, pand U, a human monocyte-like call line Koren et al. Thus, it is contemplated that the in vivo administration of peptide esters or amides of the present invention will be of benefit in preventing allograft rejection. Vp As shown by the data displayed in Table 8 [3 H]Leu-Leu-OMe was found to be metabolized differently within various cell populations.
Each dipeptide methyl ester was assessed in a minimum of three experiments. The methyl esters of a variety of dipeptides were synthesized and analyzed for the capacity to deplete NK cell function.
The method of claims 1 or 2 wherein the cells being deactivated are within an animal and the animal is administered a biologically effective amount of the dipeptide alkyl ester. Following 51 Cr labeling, wells were thoroughly washed and varying concentrations of Leu-Leu-OMe added in 50 microL PBS and the plates incubated for 15 minutes at room temperature.
Preferable amino acids are leucine, phenylalanine, valine, isoleucine, alanine, proline, glycine or aspartic acid beta methyl ester. Preferred substituted dipeptide amides of the present invention include those having an alkyl, aryl, aralkyl or alkaryl substituent. VpKatz J. The NK toxicity of both aspartyl phenylalanine methyl ester and beta methyl aspartyl phenylalanine methyl ester was measured as described for the dipeptide methyl esters in Example 7.
Methane was used as the reagent gas for chemical ionization CI mass spectral analysis. Cells were then washed and either placed in mixed lymphocyte culture or assayed for specific lysis of K cells NK.
Vppdespite the current finding that dipeptide methyl esters containing these amino acids were potent NK toxins. The peptide esters or amides of the present invention should be usable chemotherapeutic agents for patients with natural killer cell tumors generally leukemiasalthough very few reports of these tumors are found in the literature Komiyama et al.
When T4 enriched populations of T cells were assessed, however, it was clear that even micromolar Leu-Leu-OMe caused minimal 51 Cr release from this population.
Cells were then washed, cultured at Graft versus host disease GVHD is a major problem in allogeneic bone marrow transplantation. The presently described invention relates to the discovery that dipeptide alkyl esters are cytotoxic metabolites of lysosomotropic amino acid alkyl esters.
Vp ; Perussia et al. Potential problems with these procedures include possible non-specific toxicity of therapeutic dipeptide alkyl esters and the re-emergence of NK activity from precursors not sensitive to therapeutic dipeptide alkyl esters.
Thus, it is reasonable that similar mechanisms of cytotoxic lymphocyte damage to liver cells may be occurring, and therefore benefit from therapy with peptide esters or amides of the present invention should also occur in such disease states.
When T4 enriched populations of T cells were assessed, however, it was clear that even micromolar Leu-Leu-OMe caused minimal 51 Cr release from this population. Another peptide of the series, cordyheptapeptide B, was isolated from a fungal strain Cordyceps sp.
These studies have found no significant evidence that aspartame causes cancer in animals, damages the genome, or causes cancer in humans at doses currently used.
L-leucine methyl ester Leu-OMe has previously been used as a lysosomotropic agent Thiele et al. Cordyheptapeptide A differs from the cordyheptapeptide B in one amino acid moiety i. Immunol Vpp Natural killing against K target cells was assessed by a 3 hour 51 Cr release assay and percent specific lysis calculated as previously described Thiele et al.
Aralkyl or aryl derivatives, for example benzyl and napthyl may be particularly effective.DL-Phenylalanine methyl ester hydrochloride, is an aminoacid which is widely used in pharmaceuticals and foods. Methyl L-phenylalaninate | C10H14ClNO2 | CID - structure, chemical names, physical and chemical properties, classification, patents, literature, biological.
Methyl L-phenylalaninate | C10H14ClNO2 | CID - structure, chemical names, physical and chemical properties, classification, patents, literature, biological. Synthesis And Nmr Study Of Dipeptide The Methyl Ester Of N Acetyl L Prolyl L Phenylalanine experiment synthesized H-Gly-Phe-OH dipeptide using “Fmoc chemistry”.
The first part of experiment was the synthesis of L -. P - L-Phenylalanine methyl ester hydrochloride EMAIL THIS PAGE TO A FRIEND.
To Email: From Email: Message: P Aldrich L-Phenylalanine methyl ester hydrochloride 98% CAS Number Linear Formula C 6 H 5 CH 2 CH(NH 2)COOCH 3 · HCl. Molecular Weight experiment synthesized H-Gly-Phe-OH dipeptide using “Fmoc chemistry”.
The first part of experiment was the synthesis of L-phenylalanine methyl ester hydrochloride.
The methyl ester can be synthesised by reaction of thionyl chloride, SOCl2 and dry methanol with L-phenylalanine under reflux condition.Download